Primer

Part:BBa_K4449008:Design

Designed by: SRIJANI SAHA , RASHMI RANJAN BEHERA , SATYAM KUMAR SINGH   Group: iGEM22_IISER_Berhampur   (2022-10-04)

Design

The primer was designed using SnapGene and the primer parameters were checked by Oligo Evaluator ™ ( Sigma Aldrich)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]